Efficacy of Autologous Platelet-Rich Plasma (PRP) Activated By Thromboplastin-D on the Repair and Regeneration of Wounds in Dogs

ObjectiveTo evaluate the effects of autologous platelet-rich plasma (PRP) on wound healing in dogs. DesignExperimental in-vivo study. Animals5 mix-breed male adult dogs. ProceduresUnder general anesthesia, six 2 × 2-cm, full-thickness skin wounds were created on the back of dogs symmetrically. In each dog, three right side wounds were treated topically with 1.5 ml PRP jelly, whereas left side wounds received no treatment. For macroscopic evaluation, at days 0, 3, 5, 7, 10, 13, 17, 20, and 24, digital photographs were taken from wounds. At days 10, 17 and 24 after wounding, skin biopsies were taken from the center and corner of each wound for hydroxyprolin measurement and histopathologic evaluation respectively. ResultsNo statistically significant differences were found in percentage of wound contraction, epithelialization and healing between test and control group during study (P>0.05). There were no significant differences between median of hydroxyprolin levels, median of inflammatory cells infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition and collagen bundle formation scores, in the specimens from left and right wounds (P>0.05). Conclusion and Clinical RelevanceTopical application of platelet-rich plasma can not accelerate repair of the small granulating wounds of dogs. Key WordsDog, Wound healing, Plateletrich plasma, Growth factors.  Corresponding author: Hossein Kazemi Mehrjerdi, DVSc Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. E-mail address: [email protected]